10Z-Hymenialdisine Inhibits Angiogenesis by Suppressing NF-κB Activation in Pancreatic Cancer Cell Lines

Introduction: 10Z-Himenialdicin is a natural product derived from the marine sponge Axinella carteri. It has reported that an anti-angiogenetic effect through NF-κB which produces angiogenic factors such as VEGF and IL-8. Although there no previous report revealed effects of on cancer cells, potential inhibiting prevent metastasis in pancreatic cancer. Method: We investigated cell lines following experiments. 1: Colony formation assay, Cell proliferation assay. 2: Immunocyte chemistry NF-κB, Protein extraction activity assays. 3: Analysis IL-8 level by RT-PCR ELISA. 4: Tube 5: In vivo Result: initially confirmed 10Z-Hymenialdisine significantly inhibited cells. Next, we demonstrated suppressed expression both using Immunohistochemistry lines. also showed tube EA.hy926 vivo, growth (BxPC-3) were subcutaneously injected to mice model. Conclusion: As conclusion, our study antiangiogenic suppressing The novel therapeutic agent for